Antigen: Ala-Lys-Ala-Ala-Ala-Lys-Ala-Ala-Ala-Lys-Tyr peptide covalently linked to thyroglobulin. Represents a repeat sequence of elastin that becomes the desmosine crosslinks. Is not present in mature, cross-linked elastin.
Host: Rabbit.
FormSupplied as lyophilized 1 ml aliquotes of whole antiserum.
There is no species specificity.Dilute 1/100 for immunohistochemistry and 1/2000 for microtiter plate assays.
Rehydration and Storage:
Dilute to the volume indicated on the label with deionized water. Dilution with 50% glycerol, however, is preferred. This procedure eliminates the need for freezing, since 50% glycerol is an effective biological inhibitor when the product is stored at 4°C. At most working dilutions, the level of glycerol is too small to influence most assays.
Note: Variations in temperature due to freeze-thaw cycles may cause loss of activity when rehydrated product is stored in aliquots less than 50µl.