DESCRIPTION
Whole serum obtained from rabbits immunized with amino-terminal half of mouse MAGP-1.
FORM
Lyophilized. Store at 4°C until rehydrated. Stable for a minimum of 1 year at 4°C. No preservatives have been added. Additional biological protection may be provided with 0.01% thimerosal. Non-sterile.
Rehydration and Storage:
Dilute to the volume indicated on the label with deionized water. Dilution with 50% glycerol, however, is preferred. This procedure eliminates the need for freezing, since 50% glycerol is an effective biological inhibitor when the product is stored at 4°C. At most working dilutions, the level of glycerol is too small to influence most assays.
Note: Variations in temperature due to freeze-thaw cycles may cause loss of activity when rehydrated product is stored in aliquots less than 50µl.
Specificity:
Tested by Western blot and immunoprecipitation. Antibody reacts strongly with mouse MAGP-1 and show limited cross-reactivity with human and bovine MAGP-1. No reactivity with MAGP-2.
Suggested Working Dilutions:
Different assay conditions require that serial dilution of all reagents be performed to determine optimal working concentrations. Prepare working dilution immediately before use. Storage at a working dilution may result in performance loss. Suggesting starting dilutions are as follows:
- Microwell Plate Assays 1:1000 - 1:2000
- Western blots 1:200 - 1:500
- Immunoprecipitation 1:100 - 1:200
- Histo/Cytochemistry 1:50 - 1:100
ETCHING to ENHANCE ANTIGENICITY
In many cases, antigenicity of elastic fiber components can be enhanced by treating fixed tissue sections or fixed cell preparations with with 6 M guanidine HCl, 50 mM dithiothreitol, 20 mM Tris pH 8.0 for 15 minutes. After washing with 20 mM Tris pH 8.0, the slides are treated with 100 mM iodoacetamide in the dark for 15 minutes, washed, and stained using normal protocols.