Isoamylphosphonyl-Glycyl-Prolyl-L-Alamine, dipotassium salt. F.W. 454
Prepared by the methodof Galardy and Grobelny(26). Puritey is determined by thin layer chromatography and NMR. The purity is 95%. A 1 µM concentration completely inhibits 20 µM concentration completely inhibits 20µM of collagenase from Clostridium histolyticum (Ki = 20 µM). The inhibitor can be coupled to aminohexyl-agarose by reaction with EDC to yield an affinity gel (28) suitable for purification of collagenase (27).
Molecular Weight = 453.57
Exact Mass =453
Molecular Formula = C15H26K2N306P
Molecular Composition = 39.72% H 5.78% K 17.24% N 9.2% O 21.16% P 6.83%