Prepared from mouse lung by pepsin-acid extraction, salt precipitation, re-solubilization in acetic acid and re-precipitation by dialysis against neutral phosphate buffer. The collagen is re-solubilized and lyophilized. Salt-free.
Electrophoresis in 5% SDS polyacrylamide indicates high purity with typical migration of gamma, beta(2), alpha 1 and alpha 2 bands.