Prepared from calf skin collagen (EPC Product No. C857) by labeling wth fluorescein-isothicyanate (isomer 1).
Excess FITC is removed by gel filtration and sequential precipitations with NaCl and dialysis (43).
3-4 mg collagen fluorescein per ml.
The collagen fluorescein is dissolved 0.1 to 4 mg/ml in 0.02 M aceitic acid at 5°C for 18 � 24 hours. Then collagen fluorescein is dialyzed at 5°C vs. 0.1 M Tris pH 7.5 containing 0.2 M NaCl. We follow the procedure of Baici et al., Anal. Biochem., Vol 108, page 230 (1980).
Store at 5°C. DO NOT FREEZE