EC 3.1.1.3
SOURCE. Porcine pancreas.
SPECIFICATIONS.
Purified , lyophilized powder.
Standardized with lactose.
Stable 9-12 months at 5°C.
Stable 1-2 years at -20°C.
16,000 units per gram of powder.
One unit releases 100 micromoles of fatty acid per hour from olive oil emulsion at pH 7.8 and 37°C.
See assay procedure described in Methods.
Alpha amylase activity 0.02-0.05 units per mg of powder at 37°C.
PREPARATION.
Lipase is purified by modifications of the method of Verger et al. (31) to a specific activity of 120,000-145,000 units per gram of lyophilized protein.
The purified lipase is diluted with dry lactose so that the final preparation contains 16,000 units per gram (approx. 12% protein).