EC 3.4.21.4
SOURCE. Rat pancreas.
SPECIFICATIONS.
From atuolyzed rat pancreas.
Chromatographically purified, lyophilized, salt-free.
E,1% 280= 14.50. MW-25,000
ACTIVITY.
15-20 units per mg of protein. One unit will hydrolyze 1 µmole of N-Benzoyl-Phe-Val-Arg-pNA (EPC No. NB32) per minute at pH 8.3 and 25°C. Assay buffer is 0.10 M Tris pH 8.3 containing 10 mM CaCI2 and 0.2 mM substrate. Use 1-5 µg enzyme in the assay.
Chymotrypsin;<.01% of specific activity on the substrate
Suc-Al-Al-Pro-Phe-pNA.(EPC No. FH237).
Assay buffer is 0.10 M Tris pH 8.3 containing 10 mM CaCl2and 0.2mM substrate.