EC 3.4.21.4
SOURCE. Rat Pancreas.
SPECIFICATIONS. From autolyzed rat pancreas. Chromatographically purified, lyophilized, salt-free. E, 1%, 280 nm=14.50 MW= 25000
Store at 5°C. Stable 9 to 12 months at 5°C.
ACTIVITY 15-20 units per mg of protein. One unit will hydrolyze 1 µM of N-benzoyl-Phe-Val-Arg-pNA (EPC no. NB32) per minute at pH 8.3 and 25°C. Assay buffer is 0.10 M Tris pH 8.3 containing 10 mM CaCl2 and 0.2 mM substrate.
Use 1-5 µg enzyme in the assay.
Chymotrypsin; >0.1% of specific activity on the assay N-Suc-Al-Al-Pro-Phe-pNA (EPC No. SG554). Assay buffer is 0.10 M Tris pH 8.3 containing 10 mM CaCl2 and 0.2 mM substrate.