Antigen: Human leucocyte elastase.
Host: Rabbit
Form:
Lyophilized. Store at 4°C until rehydrated. Stable for a minimum of 1 year at 4°C. No preservatives have been added. Additional biological protection may be provided with 0.01% thimerosal. Non-sterile.
Rehydration and Storage:
Dilute to the volume indicated on the label with deionized water. Dilution with 50% glycerol, however, is preferred. This procedure eliminates the need for freezing, since 50% glycerol is an effective biological inhibitor when the product is stored at 4°C. At most working dilutions, the level of glycerol is too small to influence most assays.
Note: Variations in temperature due to freeze-thaw cycles may cause loss of activity when rehydrated product is stored in aliquots less than 50µl.
Specificity:
Tested by immunohistology as well as ELISA and Western blot techniques (5, 57). This product reacts most strongly with human neutrophil elastase. Cross-reactivity with elastase from non-human species has not been determined.
Suggested Working Dilutions:
Different assay conditions require that serial dilution of all reagents be performed to determine optimal working concentrations. Prepare working dilution immediately before use. Storage at a working dilution may result in performance loss. Suggesting starting dilutions are as follows:
- Microwell Plate Assays 1:1000 - 1:2000
- Western blots 1:200 - 1:500
- Immunoprecipitation 1:100 - 1:200
- Histo/Cytochemistry 1:50 - 1:100